소목 추출물의 구강암 및 골육종 세포주에 대한 항암작용에 관한 연구
ANTICANCER EFFECTS OF CAESALPINIA SAPPAN EXTRACTS ON
이종수, 김정희, 김여갑,
소속 상세정보
이종수 ( Lee Jong-Su ) - 경희대학교 치과대학 구강악안면외과학교실
김정희 ( Kim Jeong-Hee ) - 경희대학교 치과대학 구강생화학교실
김여갑 ( Kim Yeo-Gap ) - 경희대학교 치과대학 구강악안면외과학교실
KMID : 0355620070330060583
Abstract
Anticancer effect of methanol extract of Caesalpinia sappan L. on oral carcinoma (KB) and osteosarcoma (HOS) cells were investigated in this study.In order to elucidate the anticancer mechanism of Caesalpinia sappan L, we analyzed telomerase inhibitory effect of the methanol extract of Caesalpinia sappan L. In addition, we prepared 5 fraction samples according to its polarity differences and analyzed anticancer effects on oral carcinoma and osteosarcoma cells. Following results are obtained in this study.
1. 50% cell proliferation inhibitory value (IC50) of the methanol extract of Caesalpinia sappan L. against oral carcinoma (KB) cells and osteosarcoma (HOS) cells were 9.0 ㎍/㎖ and 10.9 ㎍/㎖, respectively.
2. The methanol extract of Caesalpinia sappan L. showed inhibitory effect of telomerase which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Caesalpinia sappan is at least partially due to telomerase inhibitory effect.
3. Five fraction samples were prepared according to its polarity and 88.7% of ingredient of total methanol extract was transferred to ethylacetate fraction. Thin layer chromatography analysis showed that dichloromethane fraction contained ingredient with relatively high polarity and ethylacetate fraction contained similar ingredient found in total methanol extract.
4. Anticancer effect was observed in n-hexane, dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had IC50 value of 4.4 ㎍/㎖ and > 4.0 ㎍/㎖ against oral carcinoma (KB) cells and osteosarcoma (HOS) cells, respectively.
키워드
Caesalpinia sappan extract;Oral carcinoma;Psteosarcoma;Telomerase
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